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Rapid Blue-Carba test: reduction in the detection time of carbapenemases performed from a 4-hour bacterial lawn.

Authors
  • Nastro, Marcela1
  • Ayora, Melisa1
  • García, Susana1
  • Vay, Carlos1
  • Famiglietti, Ángela1
  • Rodriguez, Carlos Hernán1
  • 1 a Laboratorio de Bacteriología, Facultad de Farmacia y Bioquímica, Departamento de Bioquímica Clínica , Hospital de Clínicas José de San Martín, Universidad de Buenos Aires , Buenos Aires , Argentina.
Type
Published Article
Journal
Journal of chemotherapy (Florence, Italy)
Publication Date
June 2017
Volume
29
Issue
3
Pages
150–153
Identifiers
DOI: 10.1080/1120009X.2016.1201272
PMID: 27347714
Source
Medline
Keywords
License
Unknown

Abstract

The increase in carbapenem-resistant gram-negative bacteria is a matter of concern due to the limited therapeutic options available. In severe infections caused by these isolates, the rapid detection of the mechanisms of resistance is vital. We described a slightly modified version of the Blue-Carba test, rapid Blue-Carba test, which allows the detection of carbapenemases at 4 h of incubation from a haze of bacterial growth obtained from a positive blood culture. It was able to detect carbapenemase-producing isolates (Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii) with a sensitivity and specificity of 98.1 and 100%, respectively. It is a rapid, easy-to-perform and an inexpensive technique that can be applied to routine laboratories, together with the simultaneous identification by mass spectrometry which would help to screen non-enzymatic carbapenem resistance; this method allows the detection of clinically relevant multidrug-resistant bacteria and the early implementation of accurate therapeutic interventions.

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