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Rapid 3D bioprinting of decellularized extracellular matrix with regionally varied mechanical properties and biomimetic microarchitecture.

Authors
  • Ma, Xuanyi1
  • Yu, Claire2
  • Wang, Pengrui3
  • Xu, Weizhe1
  • Wan, Xueyi4
  • Lai, Cheuk Sun Edwin5
  • Liu, Justin3
  • Koroleva-Maharajh, Anna2
  • Chen, Shaochen6
  • 1 Department of Bioengineering, University of California, San Diego, La Jolla, CA, 92093, USA.
  • 2 Department of NanoEngineering, University of California, San Diego, La Jolla, CA, 92093, USA.
  • 3 Materials Science and Engineering Program, University of California, San Diego, La Jolla, CA, 92093, USA.
  • 4 Division of Biological Sciences, University of California, San Diego, La Jolla, CA, 92093, USA.
  • 5 Chemical Engineering Program, University of California, San Diego, La Jolla, CA, 92093, USA.
  • 6 Department of Bioengineering, University of California, San Diego, La Jolla, CA, 92093, USA; Department of NanoEngineering, University of California, San Diego, La Jolla, CA, 92093, USA; Materials Science and Engineering Program, University of California, San Diego, La Jolla, CA, 92093, USA; Chemical Engineering Program, University of California, San Diego, La Jolla, CA, 92093, USA. Electronic address: [email protected]
Type
Published Article
Journal
Biomaterials
Publication Date
Dec 01, 2018
Volume
185
Pages
310–321
Identifiers
DOI: 10.1016/j.biomaterials.2018.09.026
PMID: 30265900
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Hepatocellular carcinoma (HCC), as the fifth most common malignant cancer, develops and progresses mostly in a cirrhotic liver where stiff nodules are separated by fibrous bands. Scaffolds that can provide a 3D cirrhotic mechanical environment with complex native composition and biomimetic architecture are necessary for the development of better predictive tissue models. Here, we developed photocrosslinkable liver decellularized extracellular matrix (dECM) and a rapid light-based 3D bioprinting process to pattern liver dECM with tailorable mechanical properties to serve as a platform for HCC progression study. 3D bioprinted liver dECM scaffolds were able to stably recapitulate the clinically relevant mechanical properties of cirrhotic liver tissue. When encapsulated in dECM scaffolds with cirrhotic stiffness, HepG2 cells demonstrated reduced growth along with an upregulation of invasion markers compared to healthy controls. Moreover, an engineered cancer tissue platform possessing tissue-scale organization and distinct regional stiffness enabled the visualization of HepG2 stromal invasion from the nodule with cirrhotic stiffness. This work demonstrates a significant advancement in rapid 3D patterning of complex ECM biomaterials with biomimetic architecture and tunable mechanical properties for in vitro disease modeling. Copyright © 2018. Published by Elsevier Ltd.

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