A low pressure plasma process based on plasma deposition has been used todevelop a drug delivery strategy. In this study, a drug delivery system based ondifferent layers of plasma co-polymerized Poly ε-caprolactone-Polyethylene glycol(PCL-PEG) co-polymers was deposited on biocompatible substrates. Cis-platinum(118 μgm/cm2) was used as an anti-cancer drug and incorporated for local deliveryof the chemotherapeutic agent. The co-polymer layers and their interaction withcancer cells were analyzed by scanning electron microscopy. Our study showed thatthe plasma-PCL-PEG coated cellophane membranes, in which the drug, was includeddid not modify the flexibility and appearance of the membranes. This system wasactively investigated as an alternative method of controlling localized delivery of drugin vivo. The loading of the anti-cancer drug was investigated by UV-VIS spectroscopyand its release from plasma deposited implants against BALB/c mice liver tissueswere analyzed through histological examination and apoptosis by TUNEL assay. Thehistological examination of liver tissues revealed that when the plasma-modifiedmembranes encapsulated the cis-platinum, the Glisson’s capsule and liver parenchymawere damaged. In all cases, inflammatory tissues and fibrosis cells were observedin contact zones between the implant and the liver parenchyma. In conclusion, lowpressure plasma deposited uniform nano-layers of the co-polymers can be used forcontrolled release of the drug in vivo.