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RACK1 promotes the proliferation of THP1 acute myeloid leukemia cells.

Authors
  • Zhang, Dalin
  • Wang, Qingyang
  • Zhu, Ting
  • Cao, Junxia
  • Zhang, Xueying
  • Wang, Jing
  • Wang, Xiaoqian
  • Li, Yan
  • Shen, Beifen
  • Zhang, Jiyan
Type
Published Article
Journal
Molecular and Cellular Biochemistry
Publisher
Springer-Verlag
Publication Date
Dec 01, 2013
Volume
384
Issue
1-2
Pages
197–202
Identifiers
DOI: 10.1007/s11010-013-1798-0
PMID: 24000012
Source
Medline
License
Unknown

Abstract

The receptor for activated C kinase 1 (RACK1), an adaptor protein implicated in the regulation of multiple signaling pathways, has been reported to contribute to the survival of leukemic progenitor cells by enhancing the activity of glycogen synthase kinase 3β (GSK3β). However, it remains unknown whether RACK1 also contributes to the oncogenic growth of acute myeloid leukemia (AML) cells. Here, we report that transient or stable silencing of endogenous RACK1 expression by RACK1 short hairpin RNAs (shRNAs) led to impaired proliferation of THP1 AML cells without inducing terminal differentiation. Further exploration revealed that RACK1 loss-of-function resulted in reduced GSK3β activity. GSK3β shRNA treatment showed similar effects to RACK1 loss-of-function. Our data collectively suggest that RACK1 contributes to THP1 cell proliferation through, at least partially, enhancing GSK3β activity. Thus, targeting RACK1 may have some important therapeutic implications in the treatment of AML.

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