Affordable Access

Quinolinic acid is produced by macrophages stimulated by platelet activating factor, Nef and Tat.

Authors
  • Smith, D G
  • Guillemin, G J
  • Pemberton, L
  • Kerr, S
  • Nath, A
  • Smythe, G A
  • Brew, B J
Type
Published Article
Journal
Journal of neurovirology
Publication Date
Feb 01, 2001
Volume
7
Issue
1
Pages
56–60
Identifiers
PMID: 11519483
Source
Medline
License
Unknown

Abstract

Activated macrophages produce quinolinic acid (QUIN), a neurotoxin, in several inflammatory brain diseases including AIDS dementia complex. We hypothesized that IL1-beta, IL6, transforming growth factor (TGF-beta2 and platelet activating factor could increase macrophage QUIN production. And that the HIV-1 proteins Nef, Tat and gp41 may also increase synthesis of QUIN by macrophages. At 72 h there were significant increases in QUIN production in the cells stimulated with PAF (914 +/- 50 nM) and Nef (2781 +/- 162 nM), with somewhat less production by Tat stimulation (645 +/- 240 nM). The increases in QUIN production approximated in vitro concentrations of QUIN shown to be neurotoxic and correlated closely with indoleamine 2,3-dioxygenase induction. IL1-beta, IL6, TGF-beta2 and gp41 stimulation produced no significant increase in QUIN production. These results suggest that some of the neurotoxicity of PAF, nef and tat may be mediated by QUIN.

Report this publication

Statistics

Seen <100 times