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Quantum dots-based sandwich immunoassay for sensitive detection of Alzheimer's disease-related Aβ1-42.

Authors
  • Tang, Menghuan1
  • Pi, Jiangli1
  • Long, Yijuan1
  • Huang, Ning2
  • Cheng, Yuan2
  • Zheng, Huzhi3
  • 1 Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. , (China)
  • 2 Department of Neurosurgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, PR China. , (China)
  • 3 Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
Publication Date
Aug 05, 2018
Volume
201
Pages
82–87
Identifiers
DOI: 10.1016/j.saa.2018.04.060
PMID: 29734108
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Amyloid-beta peptide 1-42 (Aβ1-42) is known as a component of amyloid plaques in association with Alzheimer's disease. Herein, we developed a reliable and remarkably sensitive sandwich immunoassay to detect the Aβ1-42 using quantum dots (QDs) as fluorescent label. In the presence of Aβ1-42, the biotinylated Anti-beta Amyloid 1-16 (N-Ab) recognized the target and formed C-Ab-Aβ1-42-N-Ab sandwich immunocomplexes. Then Streptavidin-QDs conjugated to biotinylated N-Ab and the concentration of Aβ1-42 was determined by detecting the fluorescence intensity in the supernatant. This method is faster and more efficient than the previous approach we reported. It also has reasonable sensitivity and selectivity. Under the optimized conditions, the linear range is 5.0 to 100 pM (0.023-0.45 ng/mL) and the detection limit is 1.7 pM (7.6 pg/ mL). In addition, this method has been successfully applied to detect the Aβ1-42 in human cerebrospinal fluid sample. Copyright © 2018 Elsevier B.V. All rights reserved.

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