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Quantitative Structure-Activity Relationship of Humic-Like Biostimulants Derived From Agro-Industrial Byproducts and Energy Crops.

  • Savy, Davide1
  • Brostaux, Yves2
  • Cozzolino, Vincenza3, 4
  • Delaplace, Pierre1
  • du Jardin, Patrick1
  • Piccolo, Alessandro3, 4
  • 1 Plant Sciences, Gembloux Agro-Bio Tech, University of Liège, Liège, Belgium. , (Belgium)
  • 2 Statistical Modelling and Development, Gembloux Agro-Bio Tech, University of Liège, Liège, Belgium. , (Belgium)
  • 3 Interdepartmental Research Centre of Nuclear Magnetic Resonance for the Environment, Agri-Food and New Materials, University of Naples Federico II, Naples, Italy. , (Italy)
  • 4 Department of Agricultural Sciences, Università di Napoli Federico II, Naples, Italy. , (Italy)
Published Article
Frontiers in Plant Science
Frontiers Media SA
Publication Date
Jan 01, 2020
DOI: 10.3389/fpls.2020.00581
PMID: 32528492


Humic-like substances (HLSs) isolated by alkaline oxidative hydrolysis from lignin-rich agro-industrial residues have been shown to exert biostimulant activity toward maize (Zea mays L.) germination and early growth. The definition of a quantitative structure-activity relationship (QSAR) between HLS and their bioactivity could be useful to predict their biological properties and tailor plant biostimulants for specific agronomic and industrial uses. Here, we created several projection on latent structure (PLS) regression by using published analytical data on the molecular composition of lignin-derived HLS obtained by both 13C-CPMAS-NMR spectra directly on samples and 31P-NMR spectra after derivatization of hydroxyl functions with a P-containing reagent (2-chloro-4,4,5,5-tetramethyl-1,3,2-dioxaphospholane). These spectral data were used to model the effect of HLS on the elongation of primary root, lateral seminal roots, total root apparatus, and coleoptile of maize. The 13C-CPMAS-NMR data suggested that methoxyl and aromatic moieties positively affected plant growth, while the carboxyl/esterified functions showed a negative impact on the overall seedling development. Alkyl C seems to promote Col elongation while concomitantly reducing that of the root system. Additionally, 31P-NMR-derived spectra revealed that the elongation of roots and Col were enhanced by the occurrence of aliphatic hydroxyl groups, and guaiacyl and p-Hydroxyphenyl lignin monomers. The PLS models based on raw dataset from 13C-CPMAS-NMR spectra explained more than 74% of the variance for the length of lateral seminal roots, total root system and coleoptile, while other parameters derived from 13C-CPMAS-NMR spectra, namely the Hydrophobicity and Hydrophilicity of materials were necessary to explain 83% of the variance of the primary root length. The results from 31P-NMR spectra explained the observed biological variance by 90, 96, 96, and 93% for the length of primary root, lateral seminal roots, total root system and coleoptile, respectively. This work shows that different NMR spectroscopy techniques can be used to build up PLS models which can predict the bioactivity of lignin-derived HLS toward early growth of maize plants. The established QSAR may also be exploited to enhance by chemical techniques the bioactive properties of HLS and enhance their plant stimulation capacity. Copyright © 2020 Savy, Brostaux, Cozzolino, Delaplace, du Jardin and Piccolo.

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