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Quantitative proteomic analysis reveals that transmissible gastroenteritis virus activates the JAK-STAT1 signaling pathway.

Authors
  • An, Kang
  • Fang, Liurong
  • Luo, Rui
  • Wang, Dang
  • Xie, Lilan
  • Yang, Jing
  • Chen, Huanchun
  • Xiao, Shaobo
Type
Published Article
Journal
Journal of Proteome Research
Publisher
American Chemical Society
Publication Date
Dec 05, 2014
Volume
13
Issue
12
Pages
5376–5390
Identifiers
DOI: 10.1021/pr500173p
PMID: 25357264
Source
Medline
Keywords
License
Unknown

Abstract

Transmissible gastroenteritis virus (TGEV), a porcine enteropathogenic coronavirus, causes lethal watery diarrhea and severe dehydration in piglets. In this study, liquid chromatography-tandem mass spectrometry coupled to isobaric tags for relative and absolute quantification labeling was used to quantitatively identify differentially expressed cellular proteins after TGEV infection in PK-15 cells. In total, 162 differentially expressed cellular proteins were identified, including 60 upregulated proteins and 102 downregulated proteins. These differentially expressed proteins were involved in the cell cycle, cellular growth and proliferation, the innate immune response, etc. Interestingly, many upregulated proteins were associated with interferon signaling, especially signal transducer and activator of transcription 1 (STAT1) and interferon-stimulated genes (ISGs). Immunoblotting and real-time quantitative reverse transcription polymerase chain reaction demonstrated that TGEV infection induces STAT1 phosphorylation and nuclear translocation, as well as ISG expression. This study for the first time reveals that TGEV induces interferon signaling from the point of proteomic analysis.

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