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Quantitative and microscopic methods for studying the endocytic pathway.

Authors
  • Rivero, Francisco
  • Maniak, Markus
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2006
Volume
346
Pages
423–438
Identifiers
PMID: 16957305
Source
Medline
License
Unknown

Abstract

Endocytosis is a process that is essential to the life of all eukaryotic cells. Laboratory strains of Dictyostelium are extremely efficient in the uptake of both particles and fluid. Many different cellular processes feed into the endocytic pathway, and many organelle-associated and cytoplasmic proteins, including the ones from the cytoskeleton, contribute to the efficiency of transit. Therefore mutants, especially in genes of unknown function, must be characterized regarding their endocytic performance. We describe the most common tools and protocols to visualize and quantify all of the individual steps in endocytic transit.

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