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Quantitative measurement of events in the mammalian unfolded protein response.

Authors
  • Shang, Jie
Type
Published Article
Journal
Methods (San Diego, Calif.)
Publication Date
Apr 01, 2005
Volume
35
Issue
4
Pages
390–394
Identifiers
PMID: 15804612
Source
Medline
License
Unknown

Abstract

The accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) triggers the unfolded protein response. IRE1, PERK, ATF6, BiP, EDEM, lipid-linked oligosaccharides (LLOs), and XBP1 directly or indirectly participate in this process. This article provides methods used in our laboratory to quantitatively measure the accumulation of mRNAs encoding BiP and EDEM; splicing of XBP-1; cleavage of ATF6; inhibition of protein synthesis by PERK; and extension of LLOs under control and stress conditions.

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