Affordable Access

Quantitative determination of soybean meal content in compound feeds: comparison of near-infrared spectroscopy and real-time PCR.

Authors
Type
Published Article
Journal
Analytical and Bioanalytical Chemistry
1618-2650
Publisher
Springer-Verlag
Publication Date
Volume
389
Issue
7-8
Pages
2313–2322
Identifiers
PMID: 17901946
Source
Medline
License
Unknown

Abstract

Standard methods for determining the raw material content of compound feed are little exploited, except for the identification of meat and bone meal in feeds. In this work, near-infrared (NIR) spectroscopy and real-time polymerase chain reaction (PCR) were applied in order to establish new and fast methods for quantification of soybean meal content in compound feeds. The best prediction quality was achieved by using a model based on NIR spectroscopy (R2 = 0.9857, standard error of cross-validation 1.1065). Furthermore, a sensitive qualitative detection method by using the real-time PCR was developed (R2 = 0.976, slope -3.7599). Finally, the differences between the real-time PCR result and the NIR spectroscopy result for a given sample were also treated, and we found that the NIR spectroscopy method provided quite accurate results which approach closely those of the real-time PCR method.

Statistics

Seen <100 times