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[Quantitative determination of protector activity of fibrinogen tryptic hydrolysates].

Authors
Type
Published Article
Journal
Ukraïns'kyĭ biokhimichnyĭ zhurnal
Publication Date
Volume
49
Issue
4
Pages
118–123
Identifiers
PMID: 19861
Source
Medline
License
Unknown

Abstract

A method is developed for the quantitative determination of the protector activity. The method is based on registrating the coagulation time shortening for a mixture of fibrin monomer and fragment D. All determinations should be carried out at high concentrations of fragment D when its inhibitory effect on fibrin polymerization is very strong. It is necessary to control the exact conditions as to ionic strength and pH of the reaction mixture. Another approach to the protector activity estimation is demonstrated. It is based on determination of clot protein quantities after fibrin monomer clotting in mixtures with constant fragment D and different protector quantities.

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