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Quantitative detection of species-specific DNA in feedstuffs and fish meals.

Authors
  • Krcmar, Pavel
  • Rencova, Eva
Type
Published Article
Journal
Journal of food protection
Publication Date
Jun 01, 2005
Volume
68
Issue
6
Pages
1217–1221
Identifiers
PMID: 15954711
Source
Medline
License
Unknown

Abstract

A sensitive and rapid method for the quantitative detection of bovine-, ovine-, swine-, and chicken-specific mitochondrial DNA sequences based on real-time PCR has been developed. The specificity of the primers and probes for real-time PCR has been tested using DNA samples of other vertebrate species that may also be present in rendered products. The quantitative detection was performed with dual-labeled probes (TaqMan) using absolute quantification with external standards of single species meat-and-bone meals. This method facilitates the detection of 0.01% of the target species-derived material in concentrate feed mixtures and fish meals.

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