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Quantitative detection of human immunodeficiency virus type 1 (HIV-1) proviral DNA in peripheral blood mononuclear cells by SYBR green real-time PCR technique.

Authors
  • Gibellini, Davide
  • Vitone, Francesca
  • Schiavone, Pasqua
  • Ponti, Cristina
  • La Placa, Michele
  • Re, Maria Carla
Type
Published Article
Journal
Journal of Clinical Virology
Publisher
Elsevier
Publication Date
Apr 01, 2004
Volume
29
Issue
4
Pages
282–289
Identifiers
PMID: 15018857
Source
Medline
License
Unknown

Abstract

The results of this study showed that our SYBR green real-time PCR is able to detect five copies of the HIV-1 genome. Moreover, our method revealed HIV-1 proviral DNA in all the 50 HIV-1 seropositive patients ( 627 +/- 1068 HIV-1 proviral DNA copies per 10(6) PBMC, with a range of 30-6300 copies), whereas no positive signal was observed in any PBMC blood donors. Our SYBR green real-time PCR represents a sensitive and useful approach that could be applied both in HIV-1 proviral DNA reservoir determination and in HAART monitoring, particularly when the HIV-1 plasmatic RNA is undetectable.

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