Affordable Access

deepdyve-link
Publisher Website

Quantitative characterization of fluorophores in multi-component nanoprobes by single-molecule fluorescence.

Authors
  • Bumb, Ambika
  • Sarkar, Susanta K
  • Wu, Xufeng S
  • Brechbiel, Martin W
  • Neuman, Keir C
Type
Published Article
Journal
Biomedical Optics Express
Publisher
The Optical Society
Publication Date
Oct 01, 2011
Volume
2
Issue
10
Pages
2761–2769
Identifiers
DOI: 10.1364/BOE.2.002761
PMID: 22025982
Source
Medline
Keywords
License
Unknown

Abstract

Multi-modal nanoparticles incorporating fluorophores are increasingly being used for medical applications. The number of fluorophores incorporated into the nanoparticles during synthesis is stochastic, leaving some nanoparticles devoid of fluorophores. Determining the number, the brightness and the photostability of the fluorophores incorporated, and the percentage of labeled nanoparticles (labeling efficiency) remains challenging. We have determined the aforementioned quantities for two synthesized multi-modal nanoparticles by exploiting the photobleaching of fluorophores at the single-molecule level using a total internal reflection fluorescence microscope. Labeling efficiency was determined by fitting the distribution of incorporated fluorophores with a statistical model and verified by independent experiments.

Report this publication

Statistics

Seen <100 times