Affordable Access

Publisher Website

Quantitative characterization of fluorophores in multi-component nanoprobes by single-molecule fluorescence.

Authors
Type
Published Article
Journal
Biomedical Optics Express
2156-7085
Publisher
The Optical Society
Publication Date
Volume
2
Issue
10
Pages
2761–2769
Identifiers
DOI: 10.1364/BOE.2.002761
PMID: 22025982
Source
Medline
Keywords
  • (160.4236) Nanomaterials
  • (170.3880) Medical And Biological Imaging

Abstract

Multi-modal nanoparticles incorporating fluorophores are increasingly being used for medical applications. The number of fluorophores incorporated into the nanoparticles during synthesis is stochastic, leaving some nanoparticles devoid of fluorophores. Determining the number, the brightness and the photostability of the fluorophores incorporated, and the percentage of labeled nanoparticles (labeling efficiency) remains challenging. We have determined the aforementioned quantities for two synthesized multi-modal nanoparticles by exploiting the photobleaching of fluorophores at the single-molecule level using a total internal reflection fluorescence microscope. Labeling efficiency was determined by fitting the distribution of incorporated fluorophores with a statistical model and verified by independent experiments.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments