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Quantitative analysis of gene expression in a single cell by qPCR.

Authors
  • Taniguchi, Kiyomi
  • Kajiyama, Tomoharu
  • Kambara, Hideki
Type
Published Article
Journal
Nature Methods
Publisher
Springer Nature
Publication Date
Jul 01, 2009
Volume
6
Issue
7
Pages
503–506
Identifiers
DOI: 10.1038/nmeth.1338
PMID: 19525960
Source
Medline
License
Unknown

Abstract

We developed a quantitative PCR method featuring a reusable single-cell cDNA library immobilized on beads for measuring the expression of multiple genes in a single cell. We used this method to analyze multiple cDNA targets (from several copies to several hundred thousand copies) with an experimental error of 15.9% or less. This method is sufficiently accurate to investigate the heterogeneity of single cells.

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