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Quantitation of porcine cytomegalovirus in pig tissues by PCR.

Authors
  • Fryer, J F
  • Griffiths, P D
  • Fishman, J A
  • Emery, V C
  • Clark, D A
Type
Published Article
Journal
Journal of clinical microbiology
Publication Date
Mar 01, 2001
Volume
39
Issue
3
Pages
1155–1156
Identifiers
PMID: 11230447
Source
Medline
License
Unknown

Abstract

A quantitative-competitive PCR for the quantification of porcine cytomegalovirus (PCMV) was developed. The virus was detected in a variety of pig organs (including potential xenotransplant donations), with viral loads ranging from <10 to 97 genome copies/microg of DNA. This assay will have significant utility for studying the activation and replication of PCMV and in swine models for allo- and xenotransplantation.

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