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Quantification of malaria antigens PfHRP2 and pLDH by quantitative suspension array technology in whole blood, dried blood spot and plasma

Authors
  • Martiáñez-Vendrell, Xavier1
  • Jiménez, Alfons1, 2
  • Vásquez, Ana3
  • Campillo, Ana4
  • Incardona, Sandra4
  • González, Raquel1, 2, 5
  • Gamboa, Dionicia6
  • Torres, Katherine6
  • Oyibo, Wellington7
  • Faye, Babacar8
  • Macete, Eusebio5
  • Menéndez, Clara1, 2, 5
  • Ding, Xavier C.4
  • Mayor, Alfredo1, 2, 5
  • 1 ISGlobal, Hospital Clínic of Barcelona, Universitat de Barcelona, Carrer Rosselló 153 (CEK building), Barcelona, 08036, Spain , Barcelona (Spain)
  • 2 Spanish Consortium for Research in Epidemiology and Public Health (CIBERESP), Madrid, Spain , Madrid (Spain)
  • 3 Universidad de Antioquia, Medellín, Colombia , Medellín (Colombia)
  • 4 FIND, Geneva, Switzerland , Geneva (Switzerland)
  • 5 Centro de Investigação em Saúde da Manhiça (CISM), Maputo, Mozambique , Maputo (Mozambique)
  • 6 Universidad Peruana Cayetano Heredia, Lima, Peru , Lima (Peru)
  • 7 University of Lagos, Idi-Aaraba, Lagos, Nigeria , Lagos (Nigeria)
  • 8 Université Cheikh Anta Diop de Dakar, Dakar, Sénégal , Dakar (Senegal)
Type
Published Article
Journal
Malaria Journal
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Jan 09, 2020
Volume
19
Issue
1
Identifiers
DOI: 10.1186/s12936-019-3083-5
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundMalaria diagnostics by rapid diagnostic test (RDT) relies primarily on the qualitative detection of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and Plasmodium spp lactate dehydrogenase (pLDH). As novel RDTs with increased sensitivity are being developed and implemented as point of care diagnostics, highly sensitive laboratory-based assays are needed for evaluating RDT performance. Here, a quantitative suspension array technology (qSAT) was developed, validated and applied for the simultaneous detection of PfHRP2 and pLDH in a variety of biological samples (whole blood, plasma and dried blood spots) from individuals living in different endemic countries.ResultsThe qSAT was specific for the target antigens, with analytical ranges of 6.8 to 762.8 pg/ml for PfHRP2 and 78.1 to 17076.6 pg/ml for P. falciparum LDH (Pf-LDH). The assay detected Plasmodium vivax LDH (Pv-LDH) at a lower sensitivity than Pf-LDH (analytical range of 1093.20 to 187288.5 pg/ml). Both PfHRP2 and pLDH levels determined using the qSAT showed to positively correlate with parasite densities determined by quantitative PCR (Spearman r = 0.59 and 0.75, respectively) as well as microscopy (Spearman r = 0.40 and 0.75, respectively), suggesting the assay to be a good predictor of parasite density.ConclusionThis immunoassay can be used as a reference test for the detection and quantification of PfHRP2 and pLDH, and could serve for external validation of RDT performance, to determine antigen persistence after parasite clearance, as well as a complementary tool to assess malaria burden in endemic settings.

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