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Quantification of isoflavones in red clover by high-performance liquid chromatography.

Authors
Type
Published Article
Journal
Journal of Chromatography B
1570-0232
Publisher
Elsevier
Publication Date
Volume
777
Issue
1-2
Pages
123–128
Identifiers
PMID: 12270205
Source
Medline
License
Unknown

Abstract

An RP-HPLC method for the determination of daidzein, genistein, formononetin and biochanin A in red clover (Trifolium pratense L.) was developed and validated. The compounds are quantified after hydrolytic extraction using an internal standard. On a base-deactivated C(18) column good separation of the analytes, also from accompanying substances, and excellent peak shape are achieved by gradient elution with aqueous sulfuric acid and acetonitrile. The method was applied to the analysis of different red clover cultivars.

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