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Quantification of IncP-1 plasmid prevalence in environmental samples.

Authors
  • Jechalke, Sven1
  • Dealtry, Simone
  • Smalla, Kornelia
  • Heuer, Holger
  • 1 Julius Kühn-Institut-Federal Research Center for Cultivated Plants, Department of Epidemiology and Pathogen Diagnostics, Braunschweig, Germany. , (Germany)
Type
Published Article
Journal
Applied and Environmental Microbiology
Publisher
American Society for Microbiology
Publication Date
Feb 01, 2013
Volume
79
Issue
4
Pages
1410–1413
Identifiers
DOI: 10.1128/AEM.03728-12
PMID: 23241977
Source
Medline
License
Unknown

Abstract

To study the role of broad-host-range IncP-1 plasmids in bacterial adaptability to irregular environmental challenges, a quantitative real-time PCR assay was developed that specifically detects the korB gene, which is conserved in all IncP-1 plasmids, in environmental samples. IncP-1 plasmid dynamics in a biopurification system for pesticide wastes were analyzed.

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