Affordable Access

Quantification of clopidogrel in human plasma by sensitive liquid chromatography/tandem mass spectrometry.

Authors
  • Nirogi, Ramakrishna V S
  • Kandikere, Vishwottam N
  • Shukla, Manoj
  • Mudigonda, Koteshwara
  • Maurya, Santosh
  • Boosi, Ravikumar
Type
Published Article
Journal
Rapid communications in mass spectrometry : RCM
Publication Date
Jan 01, 2006
Volume
20
Issue
11
Pages
1695–1700
Identifiers
PMID: 16637000
Source
Medline
License
Unknown

Abstract

A simple, sensitive and rapid high-performance liquid chromatography/positive electrospray ionization tandem mass spectrometry method was developed and validated for the assay of clopidogrel in human plasma. Following liquid-liquid extraction, the analytes were separated using an isocratic mobile phase on a reversed-phase column and analyzed by mass spectrometry in the multiple reaction monitoring mode using the respective [M+H](+) ions, m/z 322/212 for clopidogrel and m/z 264/154 for the internal standard. The assay exhibited a linear dynamic range of 5-6000 pg/mL for clopidogrel in human plasma. The lower limit of quantification was 5 pg/mL with a relative standard deviation of less than 8%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 2.5 min for each sample made it possible to analyze more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies.

Report this publication

Statistics

Seen <100 times