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Quantification of a bacterial secondary metabolite by SERS combined with SLM extraction for bioprocess monitoring.

Authors
  • Morelli, Lidia1
  • Andreasen, Sune Zoëga
  • Jendresen, Christian Bille
  • Nielsen, Alex Toftgaard
  • Emnéus, Jenny
  • Zór, Kinga
  • Boisen, Anja
  • 1 Department of Micro- and Nanotechnology, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark. [email protected] , (Denmark)
Type
Published Article
Journal
The Analyst
Publisher
The Royal Society of Chemistry
Publication Date
Nov 20, 2017
Volume
142
Issue
23
Pages
4553–4559
Identifiers
DOI: 10.1039/c7an01393k
PMID: 29114664
Source
Medline
License
Unknown

Abstract

During the last few decades, great advances have been reached in high-throughput design and building of genetically engineered microbial strains, leading to a need for fast and reliable screening methods. We developed and optimized a microfluidic supported liquid membrane (SLM) extraction device and combined it with surface enhanced Raman scattering (SERS) sensing for the screening of a biological process, namely for the quantification of a bacterial secondary metabolite, p-coumaric acid (pHCA), produced by Escherichia coli. The microfluidic device proved to be robust and reusable, enabling efficient removal of interfering compounds from the real samples, reaching more than 13-fold up-concentration of the donor at 10 μL min-1 flow rate. With this method, we quantified pHCA directly from the bacterial supernatant, distinguishing between various culture conditions based on the pHCA production yield. The obtained data showed good correlation with HPLC analysis.

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