The quality of life of military personnel represents their self-evaluation of the quality of their own mental and physical health, social relations, and the environment in which they live and work. The aim of our study was to evaluate the relationship between quality of life and anxiety levels in military personnel of the Serbian Armed Forces. The cross-sectional study included a total of 311 randomly selected professional military personnel (officers, non-commissioned officers and professional soldiers) between 23 and 53 years of age (mean, 35.3±7 years) without current mental health problems. For the purpose of this study, we used the World Health Organization Abbreviated Instrument for Quality of Life Assessment (WHOQOL-BREF) and the Beck Anxiety Inventory (BAI). The statistical analysis included parametric and non-parametric descriptive statistics. Professional military personnel showed high satisfaction with their quality of life in the categories of social relations (82.52), psychological health (82.10) and physical health (81.68), while the satisfaction scores in the category environment category were the lowest (62.77). The average value for the total BAI score was 4.83±5.66. Quality of life decreased, while anxiety increased, with increasing age of the military personnel. Higher BREF score values were associated with lower scores on the BAI questionnaires in all subscales (p<0.001). Military personnel of the Serbian Armed Forces showed a high degree of satisfaction with their own quality of life. Learning techniques to easily overcome everyday stress would reducedreduce anxiety and improve the quality of life in military personnel of the Serbian Armed Forces.