Affordable Access

deepdyve-link
Publisher Website

Qualitative screening and quantitative determination of 569 pesticide residues in honeysuckle using ultrahigh-performance liquid chromatography coupled to quadrupole-Orbitrap high resolution mass spectrometry.

Authors
  • Zhou, Heng1
  • Cao, Yi-Min1
  • Miao, Shui1
  • Lan, Lan1
  • Chen, Ming1
  • Li, Wen-Ting1
  • Mao, Xiu-Hong1
  • Ji, Shen2
  • 1 Shanghai Institute for Food and Drug Control, Shanghai 201203, China. , (China)
  • 2 Shanghai Institute for Food and Drug Control, Shanghai 201203, China. Electronic address: [email protected] , (China)
Type
Published Article
Journal
Journal of chromatography. A
Publication Date
Nov 22, 2019
Volume
1606
Pages
460374–460374
Identifiers
DOI: 10.1016/j.chroma.2019.460374
PMID: 31447205
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

A method for both qualitative screening and quantitative determination of 569 pesticides in honeysuckle was developed based on ultrahigh-performance liquid chromatography (UHPLC) coupled to quadrupole-Orbitrap high resolution mass spectrometry and an in-house executable compound database involving the theoretical masses of precursor and fragment ions and retention times. Different workflows were evaluated, validated and compared including Full MS-SIM, Full MS/ddMS2, Full MS/AIF and Full MS/DIA. For qualitative analysis, Full MS/DIA showed relatively low screening detection limits (SDLs) for most pesticides, but its MS2 identification seemed to be not reliable enough. Full MS/ddMS2 showed good repeatability of fragment ion recognition and less false positives. For quantitative analysis, the results of four workflows were comparable in terms of linearity and trueness, while Full MS-SIM and Full MS/ddMS2 gave better precision than the other two workflows for most pesticides at the spiking level of 0.01 mg/kg. Therefore, UHPLC-Full MS/ddMS2 was considered as the optimal workflow for both qualitative and quantitative analysis of honeysuckle samples. Pesticides were present in 75 of the 82 investigated samples, with concentrations ranging from 10.0 to 4116.9 μg/kg in some of the most severely contaminated samples. Copyright © 2019. Published by Elsevier B.V.

Report this publication

Statistics

Seen <100 times