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Pyruvate dehydrogenase activity and metronidazole susceptibility in Bacteroides fragilis.

Authors
Type
Published Article
Journal
The Journal of antimicrobial chemotherapy
Publication Date
Volume
11
Issue
5
Pages
393–400
Identifiers
PMID: 6874627
Source
Medline
License
Unknown

Abstract

Pyruvate dehydrogenase activity was present and comparable (giving a colour change of 202-673 od units/min/g protein in our assay system) in two clinical strains of Bacteroides fragilis exhibiting reduced sensitivity to metronidazole (MIC 2-8 mg/l at 48 h) in a resistant strain (Ingham's) of Bact. fragilis (MIC 64/mg/l) and in two sensitive strains of Bact. fragilis (MIC 0.5 mg/l). Pyruvate dehydrogenase activity was not detected in a capnocytophaga strain (sensitive to 50 micrograms disc of metronidazole), in Escherichia coli or in 'Bact. fragilis' strain (Britz) AM24s (MIC greater than 64 mg/l). Further testing of AM24s strain indicated that it was not Bact. fragilis but a facultatively anaerobic Gram-negative rod. Pyruvate dehydrogenase activity therefore, is similar in sensitive and resistant strains of Bact. fragilis and is unlikely to be related to the mechanism of resistance to metronidazole.

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