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Pyrosequencing of the DNA gyrase gene in Neisseria species: effective indicator of ciprofloxacin resistance in Neisseria gonorrhoeae.

Authors
  • E, Lindbäck
  • M, Unemo
  • M, Akhras
  • B, Gharizadeh
  • H, Fredlund
  • Nader Pourmand
  • B, Wretlind
Type
Published Article
Journal
Apmis
Publisher
Wiley (Blackwell Publishing)
Volume
114
Issue
12
Pages
837–841
Source
UCSC Nanotech biomedical-ucsc
License
Unknown

Abstract

The quinolone resistance determining region (QRDR) of the gyrA gene in ciprofloxacin-susceptible strains (n=53) and strains of Neisseria spp. with reduced susceptibility (n=70) was determined by the pyrosequencing method. Results showed that the QRDR of the gyrA gene is an effective molecular indicator of resistance to ciprofloxacin in Neisseria gonorrhoeae, and presumably in Neisseria meningitidis, but not in all other Neisseria spp. This sequence was not unique for N. gonorrhoeae and seems unsuitable for species verification of N. gonorrhoeae. However, whether it is also possible to use this region for verification depends on the specificity of the primary screening method used.

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