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Pyrosequencing of the CYP2C9 -1766T>C polymorphism as a means of detecting the CYP2C9*8 allele.

Authors
  • Patel, Shitalben R
  • Langaee, Taimour Y
  • Wong, Shan S
  • Cavallari, Larisa H
Type
Published Article
Journal
Pharmacogenomics
Publication Date
Sep 01, 2014
Volume
15
Issue
13
Pages
1717–1722
Identifiers
DOI: 10.2217/pgs.14.130
PMID: 25410896
Source
Medline
Keywords
License
Unknown

Abstract

The CYP2C9 c.449G>A (p.R150H, rs7900194) polymorphism, which confers the CYP2C9*8 allele, is common in persons of African descent and results in reduced clearance of the narrow therapeutic index drugs, warfarin and phenytoin. Because of significant homology in DNA sequence at the 449G>A locus among CYP2C genes, the 449G>A variant cannot be reliably detected via PCR-based genotyping assays that require a short PCR product, such as pyrosequencing. Herein, we propose genotyping for the CYP2C9 c.-1766T>C polymorphism via pyrosequencing as an alternative and accurate means of identifying the CYP2C9*8 allele.

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