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Pyridine extraction of nocardial acid fastness.

Authors
Type
Published Article
Journal
Applied microbiology
Publication Date
Volume
26
Issue
3
Pages
426–428
Identifiers
PMID: 4127425
Source
Medline
License
Unknown

Abstract

Several strains of Mycobacterium and Nocardia were stained for acid fastness by using the method of Kinyoun. Duplicate, glutaraldehyde-fixed smears were extracted with fresh pyridine prior to staining. The results clearly demonstrated that both in vivo- and in vitro-grown Nocardia differed from Mycobacterium by becoming nonacid fast after extraction. This pyridine extraction of nocardial acid fastness should have diagnostic and taxonomic application.

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