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Purification of radioiodinated monoclonal antibodies using a simple 'closed procedure' device.

Authors
  • Sampson, C B
  • Solanki, C
Type
Published Article
Journal
Nuclear Medicine Communications
Publisher
Ovid Technologies (Wolters Kluwer) - Lippincott Williams & Wilkins
Publication Date
Mar 01, 1991
Volume
12
Issue
3
Pages
243–247
Identifiers
PMID: 1857585
Source
Medline
License
Unknown

Abstract

An elution device has been constructed which is capable of drawing radiolabelled monoclonal antibody through a Sephadex-G chromatography column under negative pressure. Monoclonal antibodies against ovarian carcinoma and T and B lymphocytes were radioiodinated using the Chloramine-T method. Labelling efficiencies were in the range 75-85%. After passing through the chromatographic eluter no 'free' iodide was detected in the antibody samples. The apparatus was simple to use and safe to the operator.

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