Affordable Access

Purification and properties of human deoxycytidine kinase.

Authors
  • Bohman, C
  • Eriksson, S
Type
Published Article
Journal
Advances in experimental medicine and biology
Publication Date
Jan 01, 1986
Volume
195 Pt B
Pages
311–314
Identifiers
PMID: 3020911
Source
Medline
License
Unknown

Abstract

The purification of deoxycytidine kinase from human leucemic spleen reported here result in a pure protein of molecular weight 28K. The enzyme eluates during gel filtration as a dimer and the same enzyme phosphorylates both deoxycytidine, deoxyguanosine and deoxyadenosine, but with different Km and Vmax values. Our results are in agreement with earlier studies with partially purified calf thymus deoxycytidine, but clearly different from some studies on human deoxycytidine kinase.

Report this publication

Statistics

Seen <100 times