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Purification and partial characterization of a spore cortex-lytic enzyme of Clostridium perfringens S40 spores.

Authors
Type
Published Article
Journal
Bioscience, biotechnology, and biochemistry
Publication Date
Volume
59
Issue
3
Pages
514–515
Identifiers
PMID: 7766194
Source
Medline
License
Unknown

Abstract

A spore cortex-lytic enzyme was purified in an active form from the exudate of fully germinated spores of Clostridium perfringens S40. The enzyme caused attenuation of absorbance in coatless spore suspensions and phase-darkening of the spores, but had minimal activity on isolated peptidoglycan fragments. The enzyme was identified as a 31 kDa protein which is probably an N-acetylmuramyl-L-alanine amidase. The amino-terminal 15 residues of the enzyme were: VLPEPVVPEYIVVHN.

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