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Purification of oligouronides using hollow-fiber membrane functionalised with L-histidine.

Authors
  • Delattre, C
  • Michaud, P
  • Hamze, K
  • Courtois, B
  • Courtois, J
  • Vijayalakshmi, M A
Type
Published Article
Journal
Journal of Chromatography A
Publisher
Elsevier
Publication Date
Dec 16, 2005
Volume
1099
Issue
1-2
Pages
121–126
Identifiers
PMID: 16330277
Source
Medline
License
Unknown

Abstract

The pseudobioaffinity chromatography with L-histidines immobilized onto poly(ethylene-vinyl alcohol) hollow-fiber membranes was studied for a selective purification of anionic oligosaccharides. Oligoglucuronans with different degrees of polymerization and acetylation, prepared by an enzymatic degradation of bacterial polyglucuronic acid, were used as models. The adsorption and elution parameters were studied to optimise the selective adsorption. A better understanding of the physico-chemical phenomena governing this selectivity was attempted and a few hypotheses on the mechanism of selectivity are proposed.

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