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Purification of a Mycoplasma capricolum MCS4 RNA binding protein and cloning its gene.

Authors
  • Matsuda, T
  • Ando, K
  • Sasaki, T
  • Ushida, C
  • Muto, A
Type
Published Article
Journal
Nucleic acids symposium series
Publication Date
Jan 01, 1997
Issue
37
Pages
187–188
Identifiers
PMID: 9586062
Source
Medline
License
Unknown

Abstract

MCS4 RNA (125 nt in length) is one of the small stable RNAs found in Mycoplasma capricolum cells. Gel shift assay was performed with the 5' end-32P-labeled MCS4 RNA and the S100 fraction from M. capricolum to identify the RNA binding proteins. Several bands were detected above free MCS4 RNA, indicating that the RNA formed complexes with proteins and/or RNAs. One of the proteins which specifically binds to MCS4 RNA was purified. The amino acid sequence of the N-terminus revealed 60 to 80% identity to those of glyceraldehyde-3-phosphate dehydrogenases (GAPDH) from various sources.

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