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Purification and immunological characterization of recombinant antigens expressed in the form of insoluble aggregates (inclusion bodies).

Authors
Type
Published Article
Journal
Methods in molecular medicine
1543-1894
Publication Date
Volume
13
Pages
331–343
Identifiers
DOI: 10.1385/0-89603-485-2:331
PMID: 21390853
Source
Medline
License
Unknown

Abstract

In the field of clinical diagnosis the determination of specific antibodies against distinct structural or functional antigenic proteins of a given pathogen is the most commonly used diagnostic tool for the detection of infections. Although most of the established test systems still use natural antigen from different sources, the advent of nucleic acid engineering opens up the possibility of producing proteins of limited natural availability as well as designing novel proteins using in vitro mutagenesis techniques. Recombinant technology has already proven to be an excellent alternative for the production of specific antigens, that are able to improve sensitivity as well as specifity. In general, the production of recombinant antigens for diagnostic purposes is inexpensive compared to the use of purified natural antigens. The major problems associated with the setup of recombinant test systems are, of course, the identification of those antigens or antigenic determinants that guarantee a safe serological diagnosis (see Chapter 1 and Chapter 2 ) and the expression of these fragments with high efficiency.

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