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Purification and functional analysis of a Rab27 effector munc 13-4 using a semi-intact platelet dense-granule secretion assay.

Authors
  • Shirakawa, Ryutaro
  • Higashi, Tomohito
  • Kondo, Hirokazu
  • Yoshioka, Akira
  • Kita, Toru
  • Horiuchi, Hisanori
Type
Published Article
Journal
G Protein Coupled Receptors - Structure
Publisher
Elsevier BV
Publication Date
Jan 01, 2005
Volume
403
Pages
778–788
Identifiers
PMID: 16473638
Source
Medline
License
Unknown

Abstract

We have demonstrated that small GTPase Rab27 regulates dense-granule secretion in platelets. Using Rab27a affinity chromatography, we purified Munc 13-4 as a novel Rab27a interacting protein from platelet cytosol. This chapter describes the purification of Munc 13-4 and an in vitro assay system analyzing the mechanism of dense-granule secretion in platelets. The activity of Munc 13-4 is tested in this assay.

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