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Purification of a Candida albicans germ tube specific antigen.

Authors
  • Marot-Leblond, A
  • Robert, R
  • Senet, J M
  • Palmer, T
Type
Published Article
Journal
FEMS immunology and medical microbiology
Publication Date
Oct 01, 1995
Volume
12
Issue
2
Pages
127–136
Identifiers
PMID: 8589662
Source
Medline
License
Unknown

Abstract

In a previous work, Marot-Leblond et al. identified a Candida albicans germ tube-specific antigen by the use of a monoclonal antibody (mAb 3D9.3). In the present report, we used a two-step procedure to obtain a purified preparation of this antigen from a Zymolyase extract of Candida albicans germ tubes. The extract was first fractionated by gel filtration chromatography. The immunoreactive fractions were pooled, and the 3D9.3 antigen was further purified by hydrophobic interaction chromatography using a Phenyl-superose column. Analysis by SDS-PAGE, immunoblotting and Concanavalin A staining, revealed a single, polydisperse band ranging from 110 to 170 kDa. The antigen was purified 126-fold by protein content and 16.4-fold by carbohydrate content. Recovery of the antigen was 6.8% following the two-step purification.

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