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Pulmonary intravascular monocytes/macrophages in a rat model of sepsis.

Authors
  • Charavaryamath, Chandrashekhar1
  • Janardhan, Kyathanahalli S
  • Caldwell, Sarah
  • Singh, Baljit
  • 1 Immunology Research Group and Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada. , (Canada)
Type
Published Article
Journal
The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology
Publication Date
Dec 01, 2006
Volume
288
Issue
12
Pages
1259–1271
Identifiers
PMID: 17075848
Source
Medline
Language
English
License
Unknown

Abstract

Sepsis induces recruitment of neutrophils and monocytes/macrophages in the lung and enhances host susceptibility to a secondary bacterial challenge. The phenotype and functions of recruited pulmonary intravascular monocytes/macrophages (PIMMs) in sepsis remain largely unknown. Therefore, we characterized PIMM recruitment and functions in a rat model of E. coli-induced sepsis. Male Sprague-Dawley rats were injected intraperitoneally with saline (n=10) and 48 hr after the saline treatment treated intravenously with either saline (n=5) or E. coli lipopolysachharide (LPS; 1.5 microg/kg body weight; n=5). A second group of 10 rats was infected intraperitoneally with E. coli (2x10(7) CFU/100 g) followed by intravenous injection of either saline (n=5) or LPS (n=5) 48 hr after the first treatment. Rats were euthanized at 6 hr after LPS treatment. Immunocytochemistry showed more PIMMs stained with ED-1 antibody, which specifically reacts with rat monocytes/macrophages, in rats infected with E. coli compared with the controls (P<0.05). LPS treatment of E. coli-infected rats increased the numbers of PIMMs (P<0.05) and induced more inflammation compared to other groups. Immuno-electron microscopy localized TNF-alpha, IL-10, and TGF-beta2 in recruited PIMMs in rats challenged with both E. coli and LPS. ELISA on lung homogenates showed higher concentrations of TNF-alpha, IL-10, and TGF-beta2 in rats treated with both E. coli and LPS compared with those treated with only LPS or E. coli (P<0.05). We conclude that ED-1-positive PIMMs are recruited in this model of sepsis and contain TNF-alpha, IL-10, and TGF-beta2. Copyright (c) 2006 Wiley-Liss, Inc.

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