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PtKTI12 genes influence wobble uridine modifications and drought stress tolerance in hybrid poplar.

Authors
  • Wang, Hailang1
  • Xu, Chao2
  • Zhang, Youbing3
  • Yan, Xu4
  • Jin, Xiaohuan1
  • Yao, Xiaoqing1
  • Chen, Peng1
  • Zheng, Bo5
  • 1 College of Plant Science and Technology, Huazhong Agricultural University, Xueyuan Rd, Wuhan 430070, China. , (China)
  • 2 Yuan Longping High-Tech Agriculture Co. Ltd., No. 638, Heping Rd, Changsha 410001, China. , (China)
  • 3 Guangzhou Vipotion Biotechnology Co. Ltd., 5F, Building J5, No.1 Jiantashan Road, Guangzhou Science Park, Guangzhou 510663, China. , (China)
  • 4 Shacheng Middle School, Longtan W St, Zhangjiakou 075000, China. , (China)
  • 5 Key Laboratory of Horticultural Plant Biology of Ministry of Education, College of Horticultural and Forestry Sciences, Huazhong Agricultural University, Xueyuan Rd, Wuhan 430070, China. , (China)
Type
Published Article
Journal
Tree Physiology
Publisher
Oxford University Press
Publication Date
Dec 05, 2020
Volume
40
Issue
12
Pages
1778–1791
Identifiers
DOI: 10.1093/treephys/tpaa088
PMID: 32705117
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The multisubunit Elongator complex plays key roles in transcription by interacting with RNA polymerase II and chromatin modeling. Kti proteins have been identified as the auxiliary protein for the Elongator complex. However, our knowledge of Kti proteins in woody plants remains limited. In this study, in total 16 KTI gene homologs were identified in Populus trichocarpa. Among them, the two KTI12 candidates were named PtKTI12A and PtKTI12B. Although PtKTI12A and PtKTI12B were largely different in gene expression level and tissue specificity, both genes were induced by heat and drought stresses. PtKTI12A and PtKTI12B RNAi transgenic poplar plants showed reduced levels of modified nucleosides, in particular 5-carbamoylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine. Meanwhile, their tolerance to drought was improved when subjected to withdrawal of watering. Also, the protein products of PtKTI12A and PtKTI12B had similar subcellular localization and predicted tertiary structure. The results suggest that Kti12 proteins are involved in tRNA wobble uridine modification, stress response and drought stress tolerance in hybrid poplar. © The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: [email protected]

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