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Prozone effects in microscopic agglutination tests for leptospirosis in the sera of mice infected with the pathogenic Leptospira interrogans serovar Canicola

Authors
  • Shimabukuro, Fabio Hiroto
  • Costa, Veruska Maia Da
  • Silva, Rodrigo Costa da
  • Langoni, Hélio
  • Silva, Aristeu Vieira da
  • Carvalho, Lidia Raquel de
  • Domingues, Paulo Francisco
Publication Date
Aug 01, 2013
Source
Repositório Institucional UNESP
Keywords
Language
English
License
Unknown
External links

Abstract

Mice experimentally infected with a pathogenic strain of Leptospira interrogans serovar Canicola produced false negative results (prozone effect) in a microscopic agglutination test (MAT). This prozone effect occurred in several serum samples collected at different post-infection times, but it was more prominent in samples collected from seven-42 days post-infection and for 1:50 and 1:100 sample dilutions. This phenomenon was correlated with increased antibody titres in the early post-infection phase. While prozone effects are often observed in serological agglutination assays for the diagnosis of animal brucellosis and human syphilis, they are not widely reported in leptospirosis MATs.

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