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Protocol for using MULTILAYER to reveal molecular tissue substructures from digitized spatial transcriptomes

Authors
  • Moehlin, Julien1
  • Koshy, Aysis1
  • Stüder, François1
  • Mendoza-Parra, Marco Antonio1
  • 1 Génomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, University of Evry, University Paris-Saclay, 91057 Évry, France
Type
Published Article
Journal
STAR Protocols
Publisher
Elsevier
Publication Date
Sep 14, 2021
Volume
2
Issue
4
Identifiers
DOI: 10.1016/j.xpro.2021.100823
PMID: 34585159
PMCID: PMC8450297
Source
PubMed Central
Keywords
Disciplines
  • Protocol
License
Unknown

Abstract

Spatially resolved transcriptomics (SrT) allows us to explore organ/tissue architecture from the angle of the gene programs involved in their molecular complexity. Here, we describe the use of MULTILAYER to reveal molecular tissue substructures from the analysis of localized transcriptomes (defined as gexels). MULTILAYER can process low- and high-resolution SrT data but also perform a comparative analysis within multiple SrT readouts.

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