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Protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells

Authors
  • Li, Ronghui1
  • Izpisua Belmonte, Juan Carlos1
  • 1 Salk Institute for Biological Studies, La Jolla, CA 92037, USA
Type
Published Article
Journal
STAR Protocols
Publisher
Elsevier
Publication Date
Aug 13, 2021
Volume
2
Issue
3
Identifiers
DOI: 10.1016/j.xpro.2021.100745
PMID: 34430918
PMCID: PMC8371245
Source
PubMed Central
Keywords
Disciplines
  • Protocol
License
Unknown

Abstract

Extended/expanded pluripotent stem (EPS) cells can efficiently contribute to both embryonic and extraembryonic lineages in vitro and in vivo . Starting from these cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through lineage segregation and self-organization. We also provide proof of concept that EPS-blastoids can be generated from adult cells via cellular reprogramming. EPS-blastoids provide a unique platform for studying early embryogenesis.

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