A protocol for generating induced T cells by reprogramming B cells in vivo.
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Authors
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Weng, Qitong1, 2, 3, 4, 5
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Hu, Fangxiao1, 2, 3, 5
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Zhang, Mengyun1, 2, 3, 4, 5
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Dong, Yong1, 2, 3, 4, 5
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Lv, Cui1, 2, 3, 5
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Wang, Ying1, 2, 3, 4, 5
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Liu, Xiaofei1, 2, 3, 4, 5
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Wang, Jinyong1, 2, 3, 4, 5
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1
CAS key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
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(China)
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2
Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
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(China)
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3
Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University, Guangzhou, 511436, China.
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(China)
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4
University of Chinese Academy of Sciences, Beijing 100049, China.
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(China)
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5
GUANGZHOU Regenerative Medicine and Health Laboratory, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China.
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(China)
- Type
- Published Article
- Journal
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Cell regeneration (London, England)
- Publication Date
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Sep 01, 2018
- Volume
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7
- Issue
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1
- Pages
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7–15
- Identifiers
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DOI: 10.1016/j.cr.2018.05.001
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PMID: 30671224
- Source
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Medline
- Keywords
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7-Aad, 7-Aminoactinomycin D
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Apc, Allophycocyanin
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Bv, Brilliant Violet
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Car-T, Chimeric Antigen Receptor T-Cell Immunotherapy
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Dapi, 4,6-Diamidino-2-Phenylindole
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Dn, Double Negative
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Fitc, Fluorescein Isothiocyanate
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Gfp, Green Fluorescent Protein
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Hsc, Hematopoietic Stem Cells
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Hoxb5
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Ln, Lymph Node
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Pb, Peripheral Blood
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Pe, Phycoerythrin
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Percp, Peridinin Chlorophyll
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Pro/Pre-B Cells
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Retrovirus
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Sp, Single Positive
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T Cells
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Taa-Tcr-T, Tumor-Associated Antigen-Tcr-T
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Ipsc, Induced Pluripotent Stem Cells
- Language
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English
- License
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Unknown
Abstract
Obtaining T cells by reprogramming is one of the major goals in regenerative medicine. Here, we describe a protocol for generating functional T cells from Hoxb5-expressing pro/pre-B cells in vivo. This protocol includes the construction of Hoxb5 recombinant plasmids, retroviral packaging, isolation and viral transduction of pro/pre-B cells, cell transplantation, and phenotypic analysis of induced T cells. The procedure is reproducible and straightforward, providing an approach for generating induced T cells for translational research.
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
This record was last updated on 07/23/2020 and may not reflect the most current and accurate biomedical/scientific data available from NLM.
The corresponding record at NLM can be accessed at
https://www.ncbi.nlm.nih.gov/pubmed/30671224
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