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A protocol for generating induced T cells by reprogramming B cells in vivo.

Authors
  • Weng, Qitong1, 2, 3, 4, 5
  • Hu, Fangxiao1, 2, 3, 5
  • Zhang, Mengyun1, 2, 3, 4, 5
  • Dong, Yong1, 2, 3, 4, 5
  • Lv, Cui1, 2, 3, 5
  • Wang, Ying1, 2, 3, 4, 5
  • Liu, Xiaofei1, 2, 3, 4, 5
  • Wang, Jinyong1, 2, 3, 4, 5
  • 1 CAS key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China. , (China)
  • 2 Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China. , (China)
  • 3 Joint School of Life Sciences, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University, Guangzhou, 511436, China. , (China)
  • 4 University of Chinese Academy of Sciences, Beijing 100049, China. , (China)
  • 5 GUANGZHOU Regenerative Medicine and Health Laboratory, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530, China. , (China)
Type
Published Article
Journal
Cell regeneration (London, England)
Publication Date
Sep 01, 2018
Volume
7
Issue
1
Pages
7–15
Identifiers
DOI: 10.1016/j.cr.2018.05.001
PMID: 30671224
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Obtaining T cells by reprogramming is one of the major goals in regenerative medicine. Here, we describe a protocol for generating functional T cells from Hoxb5-expressing pro/pre-B cells in vivo. This protocol includes the construction of Hoxb5 recombinant plasmids, retroviral packaging, isolation and viral transduction of pro/pre-B cells, cell transplantation, and phenotypic analysis of induced T cells. The procedure is reproducible and straightforward, providing an approach for generating induced T cells for translational research.

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