Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress
- Authors
- Publication Date
- Jun 01, 2024
- Source
- eScholarship - University of California
- Keywords
- License
- Unknown
- External links
Abstract
Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic "livers in a dish." We describe strategies to induce metabolic and alcohol-associated hepatic steatosis, inflammation, and fibrosis. We outline potential applications, including using human liver spheroids for experimental and translational research and drug screening to identify potential anti-fibrotic therapies.
