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A protocol for combined Photinus and Renilla luciferase quantification compatible with protein assays

Authors
  • Hampf, Mathias
  • Gossen, Manfred
Type
Published Article
Journal
Analytical Biochemistry
Publication Date
Jan 01, 2006
Volume
356
Issue
1
Pages
94–99
Identifiers
DOI: 10.1016/j.ab.2006.04.046
Source
Elsevier
Keywords
License
Unknown

Abstract

We established a quantitative reporter gene protocol, the P/Rluc assay system, allowing the sequential measurement of Photinus and Renilla luciferase activities from the same extract. Other than comparable commercial reporter assay systems and their noncommercial counterparts, the P/Rluc assay system was formulated under the aspect of full compatibility with standard methods for protein assays. This feature greatly expands the range of applications for assay systems quantifying the expression of multiple luciferase reporters.

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