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Proteus mirabilis causing cellulitis in broiler chickens.

Authors
  • Sanches, Matheus Silva1
  • Baptista, Ana Angelita Sampaio2
  • de Souza, Marielen2
  • Menck-Costa, Maísa Fabiana2
  • Justino, Larissa2
  • Nishio, Erick Kenji3
  • Oba, Alexandre4
  • Bracarense, Ana Paula Frederico Rodrigue...5
  • Rocha, Sergio Paulo Dejato6
  • 1 Department of Microbiology, Laboratory of Bacteriology, Center of Biological Sciences, State University of Londrina, Rodovia Celso Garcia Cid PO-BOX 6001, Londrina, Paraná, 86051-980, Brazil. , (Brazil)
  • 2 Department of Preventive Veterinary Medicine, Laboratory of Avian Medicine, Agricultural Sciences Center, State University of Londrina, Londrina, Brazil. , (Brazil)
  • 3 Department of Microbiology, Laboratory of Basic and Applied Bacteriology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil. , (Brazil)
  • 4 Department of Zootechny, Laboratory of Animal Nutrition, Agricultural Sciences Center, State University of Londrina, Londrina, Brazil. , (Brazil)
  • 5 Department of Preventive Veterinary Medicine, Laboratory of Animal Pathology, Agricultural Sciences Center, State University of Londrina, Londrina, Brazil. , (Brazil)
  • 6 Department of Microbiology, Laboratory of Bacteriology, Center of Biological Sciences, State University of Londrina, Rodovia Celso Garcia Cid PO-BOX 6001, Londrina, Paraná, 86051-980, Brazil. [email protected] , (Brazil)
Type
Published Article
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Publication Date
Sep 01, 2020
Volume
51
Issue
3
Pages
1353–1362
Identifiers
DOI: 10.1007/s42770-020-00240-1
PMID: 32067208
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Given the need to understand the virulence profile of Proteus mirabilis isolates from cellulitis in broiler chickens and their ability to cause lesions, the present study aimed to characterize genotypically and phenotypically the virulence profiles of two strains of P. mirabilis isolated from cellulitis in broilers, as well as to evaluate their ability to experimentally reproduce the lesions in vivo. The strain with the highest virulence potential (LBUEL-A33) possessed mrpA, pmfA, ucaA, atfA (fimbriae), zapA, ptA (proteases), hpmA (hemolysin), and ireA (siderophore) genes, formed a very strong biofilm, and expressed the pattern of aggregative adhesion and cytotoxicity in Vero cells. The strain with the lowest virulence potential (LBUEL-A34) did not present the pmfA and ucaA genes, but expressed the pattern of aggregative adhesion, formed a strong biofilm, and did not show cytotoxicity. Both strains developed cellulitis in an animal model within 24 h post-inoculation (PI), and the degree of lesions was not significantly altered up to 120 h PI. The LBUEL-A33 strain was also inoculated in combination with an avian pathogenic Escherichia coli (APEC 046), and the lesions showed no significant changes from the individual inoculation of these two strains. Histological analysis showed that the LBUEL-A33 strain developed characteristic cellulitis lesions. Thus, both strains of P. mirabilis isolated in our study have several virulence factors and the ability to develop cellulitis in broilers.

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