Affordable Access

Proteomic analysis of prodigiosin-induced apoptosis in a breast cancer mitoxantrone-resistant (MCF-7 MR) cell line.

Authors
  • Monge, Marta
  • Vilaseca, Marta
  • Soto-Cerrato, Vanessa
  • Montaner, Beatriz
  • Giralt, Ernest
  • Pérez-Tomás, Ricardo
Type
Published Article
Journal
Investigational new drugs
Publication Date
Feb 01, 2007
Volume
25
Issue
1
Pages
21–29
Identifiers
PMID: 16633713
Source
Medline
License
Unknown

Abstract

Prodigiosin (PG) is a bacterial, red-pigmented antibiotic with immunosuppressive and apoptotic activities. To better understand its mechanisms of action, we tried to identify proteins associated with apoptosis induced by PG. For this purpose, the variation of protein expression on exposure to apoptotic concentrations of PG was examined, by high-resolution two-dimensional gel electrophoresis (2D-E), in the MCF-7 cancer cell line resistant to mitoxantrone (MCF-7-MR). Six PG apoptosis-associated protein spots were further characterized by complementary peptide mass fingerprinting and tandem mass spectrometry data obtained on a matrix-assisted laser desorption ionization-time-of-flight/time-of-flight (MALDI-TOF/TOF) mass spectrometer. The proteins identified were involved in various cellular functions, including cell defence, DNA repair and cellular organization. Our data provide novel information on cell response to PG, a new apoptotic drug with interesting anticancer activity.

Report this publication

Statistics

Seen <100 times