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A Proteoglycan-Like Molecule Offers Insights Into Ground Substance Changes During Holothurian Intestinal Regeneration.

Authors
  • Vázquez-Vélez, Gabriel E1
  • Rodríguez-Molina, José F1
  • Quiñones-Frías, Mónica C1
  • Pagán, María1
  • García-Arrarás, José E2
  • 1 Program in Developmental Biology and Medical Scientist Training Program, Baylor College of Medicine, Houston, Texas (GEV-V)Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, Texas (GEV-V)Cellular and Molecular Pathology Graduate Program, University of Wisconsin-Madison, Madison, Wisconsin (JFR-M)Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts (MCQ-F)Department of Biology, University of Puerto Rico, Río Piedras Campus, San Juan, Puerto Rico (MP, JEG-A).
  • 2 Program in Developmental Biology and Medical Scientist Training Program, Baylor College of Medicine, Houston, Texas (GEV-V)Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Houston, Texas (GEV-V)Cellular and Molecular Pathology Graduate Program, University of Wisconsin-Madison, Madison, Wisconsin (JFR-M)Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts (MCQ-F)Department of Biology, University of Puerto Rico, Río Piedras Campus, San Juan, Puerto Rico (MP, JEG-A) [email protected]
Type
Published Article
Journal
Journal of Histochemistry & Cytochemistry
Publisher
SAGE Publications
Publication Date
June 2016
Volume
64
Issue
6
Pages
381–393
Identifiers
DOI: 10.1369/0022155416645781
PMID: 27126824
Source
Medline
Keywords
License
Unknown

Abstract

Extracellular matrix remodeling is an essential component of regenerative processes in metazoans. Among these animals, holothurians (sea cucumbers) are distinguished by their great regenerative capacities. We have previously shown that fibrous collagen as well as other fibrous components disappear from the connective tissue (CT) early during intestinal regeneration, and later return as the organ primordia form. We now report on changes of the nonfibrous component of the CT. We have used Alcian Blue staining and an antibody, Proteoglycan Like-1 (PGL-1), that recognizes a proteoglycan-like antigen to identify the presence of proteoglycans in normal and regenerating intestines. Our results show that early in regeneration, the ground substance resembles that of the mesentery, the structure from where the new intestine originates. As regeneration proceeds, Alcian Blue staining and PGL-1 labeling reorganize, so that by 4 weeks the normal intestinal CT pattern is achieved. Together with our previous findings, the data suggest that CT components that might be detrimental to regeneration disappear early on, while those that might be beneficial to regeneration, such as proteoglycans, are present throughout the regenerative process.

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