Affordable Access

deepdyve-link
Publisher Website

Protective effect of surface-modified berberine nanoparticles against LPS-induced neurodegenerative changes: a preclinical study.

Authors
  • Soudi, Salma A1
  • Nounou, Mohamed I2
  • Sheweita, Salah A1, 3
  • Ghareeb, Doaa A4, 5
  • Younis, Layla K6
  • El-Khordagui, Labiba K7
  • 1 Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt. , (Egypt)
  • 2 Department of Pharmaceutical Sciences, School of Pharmacy and Physician Assistant Studies (SOPPAS), University of Saint Joseph (USJ), Hartford, CT, 06103, USA. [email protected]
  • 3 Department of Clinical Biochemistry, Faculty of Medicine, King Khalid University, Abha, Kingdom of Saudi Arabia. , (Saudi Arabia)
  • 4 Biological screening and preclinical trial laboratory, Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, Egypt. , (Egypt)
  • 5 Pharmaceutical and Fermentation Industries Development Center, City for Scientific Research and Technology Applications, Alexandria, Egypt. , (Egypt)
  • 6 Department of Pathology, Faculty of Medicine, Alexandria University, Alexandria, Egypt. , (Egypt)
  • 7 Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, 21521, Egypt. , (Egypt)
Type
Published Article
Journal
Drug delivery and translational research
Publication Date
Oct 01, 2019
Volume
9
Issue
5
Pages
906–919
Identifiers
DOI: 10.1007/s13346-019-00626-1
PMID: 30868509
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Berberine (BBR) exerts documented protection against neurodegenerative disorders. However, data on the effect of nano-encapsulation on the neuroprotective effect of BBR are lacking. We investigated the effect of BBR loading into chitosan (CS) nanoparticles (NPs) and their surface modification with Tween 80 (T80), polyethylene glycol 4000 (PEG), and miltefosine (MFS) against lipopolysaccharide (LPS)-induced neurodegenerative changes in addition to hepatotoxicity in rats. BBR-NPs were prepared by ionic gelation and characterized for morphology by transmission electron microscopy (TEM), colloidal properties, and entrapment efficiency (EE%). The neuroprotective and hepatoprotective effects of a 14-day pretreatment with four BBR-NPs formulations (4 mg/kg BBR/day) by intraperitoneal (i.p.) injection were challenged by a single i.p. 4 mg/kg dose of LPS on the fifteenth day. Neuroprotective efficacy and potential toxicity of BBR-NPs relative to BBR solution were assessed biochemically and histopathologically. One-way ANOVA followed by Tukey's comparison test was used for statistical analysis. CS nano-encapsulation and surface modification of BBR-NPs altered the neuroprotective and hepatoprotective effects of BBR depending on the physicochemical and/or biological effects of BBR, CS, coating materials, and NP-related features. Similar to the prophylactic and treatment efficacy of NPs for brain delivery, safety of these nanostructures and their individual formulation components warrants due research attention.

Report this publication

Statistics

Seen <100 times