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The protective effect of fermented milk kefir on radiation-induced apoptosis in colonic crypt cells of rats.

Authors
  • Matsuu, Mutsumi
  • Shichijo, Kazuko
  • Okaichi, Kumio
  • Wen, Chun Yang
  • Fukuda, Eiichiro
  • Nakashima, Masahiro
  • Nakayama, Toshiyuki
  • Shirahata, Sanetaka
  • Tokumaru, Sennosuke
  • Sekine, Ichiro
Type
Published Article
Journal
Journal of radiation research
Publication Date
Jun 01, 2003
Volume
44
Issue
2
Pages
111–115
Identifiers
PMID: 13678339
Source
Medline
License
Unknown

Abstract

To evaluate the effect of fermented milk kefir on X-ray-induced apoptosis in the colon of rats, we examined the apoptotic index, the mean number of apoptotic cells detected by H&E staining per crypt in the colon, in control rats and kefir-pretreated rats drinking kefir for 12 days before irradiation. Apoptotic cells were confirmed by TUNEL staining, and active caspase-3 expression was studied by immunohistochemistry. The cell position of apoptotic cells and active caspase-3 positive cells were examined. The apoptotic index of kefir-treated rats was significantly (p < 0.05) decreased 2 h after 1 Gy irradiation in comparison with control rats at crypt cell positions 1-3, 5-7, 13, and 15. Active caspase-3 expression in the kefir-treated rats was also significantly (p < 0.05) reduced in comparison with control rats 2 h after 1 Gy irradiation at crypt cell positions 1-4, 13, and 15. This study indicated that kefir protects colonic crypt cells against radiation-induced apoptosis, which was most pronounced in the stem cell region of the crypt. The antiapoptotic effect of fermented milk kefir was due to the inhibition of caspase-3 activation.

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