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Protection of Killer Antiidiotypic Antibodies against Early Invasive Aspergillosis in a Murine Model of Allogeneic T-Cell-Depleted Bone Marrow Transplantation

Authors
  • Elio Cenci
  • Antonella Mencacci
  • Antonio Spreca
  • Claudia Montagnoli
  • Angela Bacci
  • Katia Perruccio
  • Andrea Velardi
  • Walter Magliani
  • Stefania Conti
  • Luciano Polonelli
  • Luigina Romani
Publisher
American Society for Microbiology
Publication Date
May 01, 2002
Source
PMC
Keywords
Disciplines
  • Biology
  • Medicine
License
Unknown

Abstract

Antiidiotypic monoclonal antibodies (MAbs) representing the internal image of a yeast killer toxin (KT) have therapeutic potential against several fungal infections. The efficacy of KT MAbs against Aspergillus fumigatus was investigated in a mouse model of T-cell-depleted allogeneic bone marrow transplantation (BMT) with invasive pulmonary aspergillosis. Mice were highly susceptible to infection at 3 days post-BMT, when profound neutropenia was observed both in the periphery and in the lungs. Treatment with KT MAbs protected the mice from infection, as judged by the long-term survival and decreased pathology associated with inhibition of fungal growth and hyphal development in the lungs. In vitro, similar to polymorphonuclear neutrophils, KT MAbs significantly inhibited the hyphal development and metabolic activity of germinated Aspergillus conidia. These results indicate that mimicking the action of neutrophils could be a strategy through which KT MAbs exert therapeutic efficacy in A. fumigatus infections.

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